Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit
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- SKU:
- PRDL00041
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- B2ZDZ3
- Sensitivity:
- 0.062ng/mL
- Range:
- 0.156-10ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- SLC11A2,DCT1,NRAMP2,Natural resistance-associated macrophage protein 2,Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
- Reactivity:
- Porcine
- Sample Type:
- Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:
Description
system_update_altDatasheet
Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit
The Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit is specifically designed for the quantitative measurement of DMT1 levels in porcine serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and precision, guaranteeing dependable and consistent results for various research purposes.DMT1 is a key protein responsible for the transport of divalent metals such as iron across cellular membranes.
Its dysregulation has been linked to disorders like anemia, neurodegenerative diseases, and iron overload conditions. By detecting and quantifying DMT1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop targeted interventions for their management.
| Product Name: | Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit |
| Product Code: | PRDL00041 |
| Size: | 96 Assays |
| Target: | DMT1 |
| Synonyms: | SLC11A2, DCT1, NRAMP2, Natural resistance-associated macrophage protein 2, Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters |
| Detection Method: | Sandwich |
| Range: | 0.156-10ng/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
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| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
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