The Porcine D-Dimer D2D ELISA Kit is specially designed for the precise measurement of D-dimer levels in porcine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an ideal choice for a variety of research purposes.D-dimer is a fibrin degradation product that is commonly used as a biomarker for clotting disorders and thrombotic events. Monitoring D-dimer levels is crucial in diagnosing and managing conditions such as deep vein thrombosis, pulmonary embolism, and disseminated intravascular coagulation.
By using the Porcine D-Dimer D2D ELISA Kit, researchers can accurately assess D-dimer levels in porcine samples, providing valuable insights into the mechanisms of clot formation and fibrinolysis in pigs. This kit is a valuable tool for studying coagulation disorders and developing new therapeutic approaches for treating thrombotic diseases in porcine models.
Product Name:
Porcine D-Dimer (D2D) ELISA Kit
Product Code:
PRDL00038
Size:
96 Assays
Target:
D2D
Synonyms:
D 2 Dimer
Detection Method:
Competition
Range:
78.125-5000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
