Phospho-IRS1 (Ser312) Antibody (PACO23897)
- SKU:
- PACO23897
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
| Antibody Name: | Phospho-IRS1 (Ser312) Antibody (PACO23897) |
| Antibody SKU: | PACO23897 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of serine 312 (A-T-S(p)-P-A) derived from Human IRS-1. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1(Phospho-Ser312) antibody. |
| Background: | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit |
| Synonyms: | HIRS-1; IRS1; Insulin receptor substrate 1 |
| UniProt Protein Function: | IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q36 Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; cytosol; nucleus Molecular Function:insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; glucose homeostasis; signal transduction; negative regulation of insulin secretion; response to insulin stimulus; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] |
| UniProt Code: | P35568 |
| NCBI GenInfo Identifier: | 547738 |
| NCBI Gene ID: | 3667 |
| NCBI Accession: | P35568.1 |
| UniProt Related Accession: | P35568 |
| Molecular Weight: | |
| NCBI Full Name: | Insulin receptor substrate 1 |
| NCBI Synonym Full Names: | insulin receptor substrate 1 |
| NCBI Official Symbol: | IRS1 |
| NCBI Official Synonym Symbols: | HIRS-1 |
| NCBI Protein Information: | insulin receptor substrate 1; IRS-1 |
| UniProt Protein Name: | Insulin receptor substrate 1 |
| Protein Family: | Isoleucine--tRNA ligase |
| UniProt Gene Name: | IRS1 |
| UniProt Entry Name: | IRS1_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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