Phospho-Cdc25b (Ser149) Antibody (PACO24346)
- SKU:
- PACO24346
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | Phospho-Cdc25b (Ser149) Antibody (PACO24346) |
| Antibody SKU: | PACO24346 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody. |
 | Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody. |
| Background: | Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. |
| Synonyms: | CDC25B |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9DBN8 |
| NCBI GenInfo Identifier: | 161621261 |
| NCBI Gene ID: | 12531 |
| NCBI Accession: | NP_001104545.1 |
| UniProt Secondary Accession: | Q9DBN8 |
| UniProt Related Accession: | Q9DBN8,P30306 |
| Molecular Weight: | 62,927 Da |
| NCBI Full Name: | M-phase inducer phosphatase 2 isoform b |
| NCBI Synonym Full Names: | cell division cycle 25B |
| NCBI Official Symbol: | Cdc25b |
| NCBI Official Synonym Symbols: | AI604853 |
| NCBI Protein Information: | M-phase inducer phosphatase 2 |
| UniProt Protein Name: | Cdc25b protein |
| UniProt Synonym Protein Names: | Cdc25b protein |
| Protein Family: | |
| UniProt Gene Name: | Cdc25b |
| UniProt Entry Name: | Q9DBN8_MOUSE |
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