Phospho-Bad (Ser155) Antibody (PACO24359)
- SKU:
- PACO24359
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
| Antibody Name: | Phospho-Bad (Ser155) Antibody (PACO24359) |
| Antibody SKU: | PACO24359 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of serine 155(R-M-S(p)-D-E) derived from Human BAD. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 293 cells using BAD (phospho-Ser155) antibody. |
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho- Ser155) antibody. |
| Background: | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. Schurmann A., Mooney A.F., Sanders L.C., Sells M.A., Wang H.G., Reed J.C., Bokoch G.M.Mol. Cell. Biol. 20:453-461(2000) Jakobi R., Moertl E., Koeppel M.A.J. Biol. Chem. 276:16624-16634(2001) |
| Synonyms: | Bbc2; AI325008 |
| UniProt Protein Function: | BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. |
| UniProt Protein Details: | Protein type:Apoptosis Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol Molecular Function:protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase binding; protein phosphatase 2B binding Biological Process: positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation |
| UniProt Code: | Q61337 |
| NCBI GenInfo Identifier: | 2493288 |
| NCBI Gene ID: | 12015 |
| NCBI Accession: | Q61337.1 |
| UniProt Related Accession: | Q61337 |
| Molecular Weight: | |
| NCBI Full Name: | Bcl2-associated agonist of cell death |
| NCBI Synonym Full Names: | BCL2-associated agonist of cell death |
| NCBI Official Symbol: | Bad |
| NCBI Official Synonym Symbols: | Bbc2; AI325008 |
| NCBI Protein Information: | bcl2-associated agonist of cell death; bcl-2-binding component 6; bcl2 antagonist of cell death; bcl-xL/Bcl-2-associated death promoter |
| UniProt Protein Name: | Bcl2-associated agonist of cell death |
| UniProt Synonym Protein Names: | Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death |
| Protein Family: | Bcl2-associated agonist of cell death |
| UniProt Gene Name: | Bad |
| UniProt Entry Name: | BAD_MOUSE |
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