Anti-PDE9A 항체의 (PACO57080)

Antibody Name:	PDE9A Antibody (PACO57080)
Antibody SKU:	PACO57080
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A protein (426-533AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: PDE9A antibody at 3.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 65, 66, 57 kDa. Observed band size: 69 kDa.
	Immunofluorescence staining of A549 cells with PACO57080 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO57080 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).

Synonyms:

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC 3.1.4.35), PDE9A

UniProt Protein Function:	PDE9A: Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily. 16 isoforms of the human protein are produced by alternative splicing.Protein type: Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.35Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: Golgi apparatus; endoplasmic reticulum; perinuclear region of cytoplasm; perikaryon; cytosolMolecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; protein binding; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activityBiological Process: cGMP-mediated signaling; metabolic process; blood coagulation; signal transduction
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O76083
NCBI GenInfo Identifier:	4505675
NCBI Gene ID:	5152
NCBI Accession:	NP_002597.1
UniProt Secondary Accession:	O76083,O75490, O75491, O95225, Q53Y40, Q5QD39, Q86SF7 Q86SI6, B2RBI5, B4DFI5
UniProt Related Accession:	O76083
Molecular Weight:	56,554 Da
NCBI Full Name:	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform a
NCBI Synonym Full Names:	phosphodiesterase 9A
NCBI Official Symbol:	PDE9A
NCBI Official Synonym Symbols:	HSPDE9A2
NCBI Protein Information:	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; phosphodiesterase PDE9A21; CGMP-specific 3',5'-cyclic phosphodiesterase type 9
UniProt Protein Name:	High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
UniProt Synonym Protein Names:
Protein Family:	High affinity cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name:	PDE9A
UniProt Entry Name:	PDE9A_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)