Oxaloacetate Assay Kit (Colorimetric/Fluorometric) (BA0139)

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SKU:: BA0139
UPC:
Product Type:: Assay
Detection Method:: Colorimetric
Detection Method:: Fluorometric
Instrument:: Microplate Reader
Sample Type:: Plasma, Serum, Tissue, Culture Media
Research Area:: TCA Cycle; Urea; Food Safety & Analysis; Plant & Environmental Stress; Clinical Chemistry

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Description

Oxaloacetate Assay Kit

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO₂ with pyruvate or phosphoenolpyruvate.

Assay Genie's Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the oxaloacetate concentration in the sample.

Applications

For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.

Key Features

Sensitive and accurate. Linear detection range: 7 to 400 uM oxaloacetate for colorimetric assays and 1 to 40 uM for fluorimetric assays.

Oxaloacetate Assay Kit Information:

Kit Includes:	Developer: 10 mL ODC Enzyme: 120 mL Dye Reagent: 120 mL Oxaloacetate Standard: Dried
Kit Requires:	Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.
Method of Detection:	OD570nm, or FL530/585nm
Detection Limit:	OD, FL: 4, 1 uM
Samples:	Plasma, serum, tissue and culture media
Species:	All
Protocol Length:	20 min
Size:	100 tests
Storage:	Store all kit components at -20 °C
Shelf Life:	6 months