NADK2 Antibody (PACO58184)
- SKU:
- PACO58184
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | NADK2 Antibody (PACO58184) |
| Antibody SKU: | PACO58184 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
| Species Reactivity: | Human, Rat, Mouse |
| Immunogen: | Recombinant Human NAD kinase 2, mitochondrial protein (16-156AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: NADK2 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 46, 32 kDa. Observed band size: 50, 46 kDa. |
 | IHC image of PACO58184 diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO58184 diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo. |
| Synonyms: | NAD kinase 2, mitochondrial (EC 2.7.1.23) (Mitochondrial NAD kinase) (NAD kinase domain-containing protein 1, mitochondrial), NADK2, C5orf33 MNADK NADKD1 |
| UniProt Protein Function: | NADK2: Belongs to the NAD kinase family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: EC 2.7.1.23Chromosomal Location of Human Ortholog: 5p13.2Cellular Component: mitochondrionMolecular Function: NAD+ kinase activity; protein homodimerization activityBiological Process: NAD metabolic processDisease: 2,4-dienoyl-coa Reductase Deficiency |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| UniProt Code: | Q4G0N4 |
| NCBI GenInfo Identifier: | 156630863 |
| NCBI Gene ID: | 133686 |
| NCBI Accession: | Q4G0N4.2 |
| UniProt Secondary Accession: | Q4G0N4,Q6UTX5, Q96NM0, B5MC93 |
| UniProt Related Accession: | Q4G0N4 |
| Molecular Weight: | 31,716 Da |
| NCBI Full Name: | NAD kinase 2, mitochondrial |
| NCBI Synonym Full Names: | NAD kinase 2, mitochondrial |
| NCBI Official Symbol: | NADK2 |
| NCBI Official Synonym Symbols: | DECRD; MNADK; NADKD1; C5orf33 |
| NCBI Protein Information: | NAD kinase 2, mitochondrial |
| UniProt Protein Name: | NAD kinase 2, mitochondrial |
| UniProt Synonym Protein Names: | Mitochondrial NAD kinase; NAD kinase domain-containing protein 1, mitochondrial |
| Protein Family: | NAD kinase |
| UniProt Gene Name: | NADK2 |
| UniProt Entry Name: | NAKD2_HUMAN |
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