The Mouse VWF (Von Willebrand Factor) ELISA Kit is specifically designed for the precise quantification of Von Willebrand Factor levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Von Willebrand Factor is a key protein involved in blood clotting and platelet adhesion, playing a critical role in hemostasis.
Dysregulation of VWF levels is associated with various bleeding disorders, making it an important biomarker for studying these conditions and developing therapeutic interventions.With the Mouse VWF ELISA Kit, researchers can easily measure VWF levels in mouse samples with confidence and precision, advancing our understanding of hemostasis and contributing to the development of potential treatments for bleeding disorders.
Product Name:
Mouse vWF (von Willebrand Factor) ELISA Kit
SKU:
MOES01614
Target:
Mouse vWF (von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VWF. You can calculate the concentration of Mouse VWF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
97-113
90-102
Average (%)
98
103
97
1:4
Range (%)
92-107
83-94
86-98
Average (%)
98
88
92
1:8
Range (%)
86-100
81-94
85-95
Average (%)
93
87
90
1:16
Range (%)
90-104
82-93
83-93
Average (%)
97
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.33
12.59
29.76
4.41
13.12
27.83
Standard deviation
0.23
0.75
1.48
0.3
0.64
1.45
C V (%)
5.31
5.96
4.97
6.8
4.88
5.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VWF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VWF in samples. No significant cross-reactivity or interference between Mouse VWF and analogues was observed.
