Mouse Visfatin Recombinant Protein (RPPB1093)
- SKU:
- RPPB1093
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Mouse
- Target:
- Visfatin
- Synonyms:
- PBEF
- Pre-B cell colony-enhancing factor
- Nicotinamide phosphoribosyltransferase NAmPRTase
- Nampt
- Source:
- Escherichia Coli
- Uniprot:
- Q99KQ4
Description
| Product Name: | Mouse Visfatin Recombinant Protein |
| Product Code: | RPPB1093 |
| Size: | 25µg |
| Species: | Mouse |
| Target: | Visfatin |
| Synonyms: | PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless 1mg/ml solution. |
| Formulation: | The protein solution contains 1x PBS pH-7.4. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECREKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERGWNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPITVATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHTVFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H |
Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
Visfatin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 1-491) containing a 20 aa His tag and having 511 amino acids. The total molecular mass is 57kDa. The Visfatin is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. Protein type: Cell cycle regulation; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cytokine; EC 2.4.2.12; TransferaseChromosomal Location of Human Ortholog: 12|12 A3Cellular Component: cell junction; cytoplasm; extracellular exosome; extracellular region; extracellular space; nuclear speck; nucleusMolecular Function: cytokine activity; drug binding; identical protein binding; nicotinamide phosphoribosyltransferase activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein homodimerization activity; transferase activity; transferase activity, transferring glycosyl groupsBiological Process: circadian regulation of gene expression; circadian rhythm; NAD biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; pyridine nucleotide biosynthetic process; rhythmic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q99KQ4 |
| NCBI GenInfo Identifier: | 257153454 |
| NCBI Gene ID: | 59027 |
| NCBI Accession: | NP_067499.2 |
| UniProt Secondary Accession: | Q99KQ4,Q8C3B5, Q9JKM0 |
| UniProt Related Accession: | Q99KQ4 |
| Molecular Weight: | Predicted Molecular Mass: 57.0kDaAccurate Molecular Mass: 59kDa as determined by SDS-PAGE reducing conditions. |
| NCBI Full Name: | nicotinamide phosphoribosyltransferase |
| NCBI Synonym Full Names: | nicotinamide phosphoribosyltransferase |
| NCBI Official Symbol: | Nampt |
| NCBI Official Synonym Symbols: | Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik |
| NCBI Protein Information: | nicotinamide phosphoribosyltransferase |
| UniProt Protein Name: | Nicotinamide phosphoribosyltransferase |
| UniProt Synonym Protein Names: | Pre-B-cell colony-enhancing factor 1 homolog; PBEF; Visfatin |
| Protein Family: | |
| UniProt Gene Name: | Nampt |
| UniProt Entry Name: |
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