The Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit is specifically designed for the quantitative detection of VIP levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VIP is a neuropeptide that plays a key role in regulating various physiological processes, including vasodilation, smooth muscle relaxation, and immune modulation.
Dysregulation of VIP levels has been implicated in a range of diseases, such as inflammatory disorders, autoimmune conditions, and neurological disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Mouse VIP ELISA Kit provides a reliable tool for researchers to investigate the role of VIP in various biological processes and disease pathologies in mouse models.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VIP. During the reaction, VIP in the sample or standard competes with a fixed amount of VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
85-101
88-104
Average (%)
106
92
96
1:4
Range (%)
89-106
91-105
97-115
Average (%)
96
98
105
1:8
Range (%)
85-96
91-105
93-105
Average (%)
90
97
99
1:16
Range (%)
85-100
88-102
95-108
Average (%)
91
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
95-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.6
92.1
385.2
45.7
89.5
349.5
Standard deviation
2.5
4.9
20.0
2.5
4.9
15.0
C V (%)
6.01
5.32
5.19
5.47
5.47
4.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes VIP in samples. No significant cross-reactivity or interference between VIP and analogues was observed.
