Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse VEGFR3/FLT4 Recombinant Protein (RPES1002)
Product Code:
RPES1002
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AI323512,Chy,Flt-4,VEGFR-3,VEGFR3
Accession:
P35917-1
Sequence:
Met 1-Glu 775
Fusion tag:
C-Fc
Activity:
1. Measured by its ability to bind human VEGF-D in functional ELISA.2. Immobilized human VEGF-C at 10 µg/mL (100 ?L/well) can bind mouse VEGFR3-Fc.The EC50 of mouse VEGFR3-Fc is 0.008 µg/mL.3. Measured by its ability to bind with mouse FIGF-His in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse FLT4 (P35917-1) extracellular domain (Met 1-Glu 775) was was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
112 kDa
AP Mol Mass:
150, 85, 65 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
