The Mouse VEGFR-2 (KDR) / Vascular Endothelial Growth Factor Receptor 2 ELISA Kit is a highly specialized tool for the accurate quantification of VEGFR-2 levels in mouse samples including serum, plasma, and tissue lysates. With exceptional sensitivity and specificity, this kit delivers precise and reliable results, making it a valuable asset for a wide range of experimental studies.VEGFR-2, also known as kinase insert domain receptor (KDR), is a key receptor involved in mediating vascular endothelial growth factor (VEGF) signaling pathways, crucial for angiogenesis and vascular development.
Understanding the role of VEGFR-2 in various biological processes and diseases such as cancer, cardiovascular disorders, and inflammatory conditions, is essential for advancing research and therapeutic interventions. This ELISA kit provides researchers with a powerful tool to investigate the expression and function of VEGFR-2 in mouse models, offering valuable insights for innovative research and potential therapeutic strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR-2/KDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGFR-2/KDR. You can calculate the concentration of Mouse VEGFR-2/KDR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
89-103
98-110
Average (%)
101
96
104
1:4
Range (%)
95-109
83-93
98-111
Average (%)
102
88
104
1:8
Range (%)
100-117
83-93
93-106
Average (%)
108
88
99
1:16
Range (%)
98-112
87-101
94-107
Average (%)
104
94
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.0
0.5
1.4
3.6
Standard deviation
0.03
0.06
0.13
0.03
0.08
0.12
C V (%)
6
4.62
3.25
6.0
5.71
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Mouse VEGFR-2/KDR and analogues was observed.
