The Mouse VEGFR-2 (KDR) CLIA Kit is a powerful tool for measuring VEGFR-2 levels in mouse serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, delivering precise and consistent results for a variety of research purposes.VEGFR-2, also known as KDR, is a key receptor involved in vascular endothelial growth factor (VEGF) signaling, contributing to the regulation of angiogenesis and vascular development. Dysregulation of VEGFR-2 activity has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, underscoring its importance as a potential therapeutic target and biomarker.
With the Mouse VEGFR-2 (KDR) CLIA Kit, researchers can gain valuable insights into VEGFR-2 expression levels and activity, facilitating the study of angiogenesis-related processes and the development of novel treatment strategies. Trust in the reliability and performance of this kit for your experimental needs.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR-2/KDR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VEGFR-2/KDR. You can calculate the concentration of Mouse VEGFR-2/KDR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
91-109
101-119
Average (%)
91
99
109
1:4
Range (%)
101-113
94-105
88-103
Average (%)
107
99
95
1:8
Range (%)
103-118
92-107
93-108
Average (%)
110
100
101
1:16
Range (%)
94-108
87-104
96-107
Average (%)
101
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.41
210.74
790.38
101.2
230.82
753.5
Standard deviation
12.84
20.55
80.62
11.59
25.74
52.29
C V (%)
12.79
9.75
10.2
11.45
11.15
6.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Mouse VEGFR-2/KDR and analogues was observed.
