Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse VCAM1 Recombinant Protein (RPES4324)
Product Code:
RPES4324
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
CD106,Vascular cell adhesion protein 1, Vcam1, L1CAM,VCMA1,Vcam-1
Accession:
NP_035823.3
Sequence:
Met 1-Glu 698
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 µg/ml with 100 ?l/well), approximately 70%-80% cells will adhere after 1 hour at RT.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
75.8 kDa
AP Mol Mass:
90-100 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
