The Mouse VCAM-1 (Vascular Cell Adhesion Molecule-1) ELISA Kit is specially designed for the precise measurement of VCAM-1 levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for a variety of research applications.VCAM-1 is a key protein involved in mediating cell adhesion and inflammation, playing a vital role in various physiological and pathological processes.
Its expression is upregulated in conditions such as atherosclerosis, inflammatory diseases, and cancer, highlighting its importance as a biomarker for studying these diseases and potentially developing targeted therapies.Overall, the Mouse VCAM-1 ELISA Kit offers researchers a reliable and effective method for quantifying VCAM-1 levels in mouse samples, providing valuable insights into the role of this protein in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VCAM-1/CD106. You can calculate the concentration of Mouse VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
100-115
93-104
Average (%)
100
106
98
1:4
Range (%)
98-110
86-100
94-109
Average (%)
104
92
102
1:8
Range (%)
94-105
84-97
94-111
Average (%)
100
90
101
1:16
Range (%)
97-115
85-96
95-112
Average (%)
105
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
88-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.3
0.5
1.0
4.4
Standard deviation
0.03
0.06
0.23
0.03
0.04
0.16
C V (%)
6
5.45
5.35
6.0
4.0
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Mouse VCAM-1/CD106 and analogues was observed.
