Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
The Mouse UCP-1 (Uncoupling Protein 1) Mitochondrial ELISA Kit is specifically designed for the accurate measurement of UCP-1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.UCP-1 is a pivotal protein involved in energy expenditure and thermogenesis, particularly in brown adipose tissue.
It plays a crucial role in regulating metabolism and body temperature, making it a key target for studies related to obesity, diabetes, and metabolic disorders. By accurately measuring UCP-1 levels, researchers can gain valuable insights into metabolic processes and potential therapeutic targets.
Product Name:
Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
SKU:
MOES01212
Target:
Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse UCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse UCP-1. You can calculate the concentration of Mouse UCP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
95-112
94-106
Average (%)
100
103
100
1:4
Range (%)
89-102
86-98
88-99
Average (%)
97
91
93
1:8
Range (%)
85-97
79-93
88-101
Average (%)
92
86
94
1:16
Range (%)
89-101
87-99
82-96
Average (%)
96
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
95-106
101
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
0.93
4.14
0.47
1.0
3.9
Standard deviation
0.02
0.04
0.16
0.03
0.06
0.13
C V (%)
4.17
4.3
3.86
6.38
6.0
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse UCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse UCP-1 in samples. No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed.
