The Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit is specifically designed for the precise measurement of TSH levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring dependable and consistent results, which makes it perfect for a variety of research purposes.Thyroid Stimulating Hormone is a key hormone that regulates the thyroid gland's function, controlling the production of thyroid hormones that affect metabolism, growth, and other essential bodily functions.
Dysregulation of TSH levels can indicate thyroid dysfunction, making it a valuable biomarker for studying thyroid-related conditions and potential treatment strategies.Overall, the Mouse TSH ELISA Kit offers researchers a reliable tool to accurately quantify TSH levels in mouse samples, aiding in the advancement of research in areas such as endocrinology, metabolism, and thyroid disorders.
Product Name:
Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
MOES01557
Target:
Mouse TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
94-110
95-108
Average (%)
96
101
103
1:4
Range (%)
102-117
81-92
93-108
Average (%)
107
87
100
1:8
Range (%)
96-110
82-96
96-109
Average (%)
103
89
101
1:16
Range (%)
98-112
83-95
95-107
Average (%)
106
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.4
7.3
36.9
4.4
7.1
39.0
Standard deviation
0.3
0.3
1.7
0.3
0.4
1.2
C V (%)
6.82
4.11
4.61
6.82
5.63
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed.
