The Mouse TYR (Tyrosinase) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of tyrosinase levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Tyrosinase is an enzyme crucial for melanin production and plays a vital role in pigmentation. Dysregulation of tyrosinase activity has been implicated in conditions such as albinism, vitiligo, and melanoma, making it an important biomarker for studying these disorders and exploring potential treatment options.
Overall, the Mouse TYR (Tyrosinase) CLIA Kit is an essential tool for researchers investigating melanin production, pigmentation disorders, and melanoma, providing accurate and precise measurements of tyrosinase levels in mouse samples.
Product Name:
Mouse TRY (Tyrosinase) CLIA Kit
SKU:
MOES00586
Target:
Mouse TRY (Tyrosinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRY. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRY, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TRY. You can calculate the concentration of Mouse TRY in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
88-99
88-103
Average (%)
100
94
95
1:4
Range (%)
86-100
90-103
92-103
Average (%)
91
96
97
1:8
Range (%)
95-106
93-111
87-103
Average (%)
100
101
94
1:16
Range (%)
96-107
92-108
100-113
Average (%)
101
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.91
273.9
949.56
120.67
297.52
918.02
Standard deviation
11.97
24.73
72.64
13.21
27.64
64.17
C V (%)
10.79
9.03
7.65
10.95
9.29
6.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TRY concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TRY in samples. No significant cross-reactivity or interference between Mouse TRY and analogues was observed.
