TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.
Product Name:
Mouse TROP2/TACSTD2 Recombinant Protein (RPES1316)
Product Code:
RPES1316
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Tumor-associated calcium signal transducer 2,Tacstd2,Trop2,Cell surface glycoprotein Trop-2, TROP-2,C80403,EGP-1,GA733-1,Ly97
Accession:
Q8BGV3
Sequence:
Met 1-Gln 270
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.When 5 x 10E4 cells/well are added to TACSTD2-coated plates (40 µg/mL, 100 ?L/well), > 50% cells will adhere after 1 hour at 37?.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3?) (Met 1-Gln 270) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
29.4 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
