The Mouse TRF (Transferrin) CLIA Kit is specifically designed for the quantitative measurement of transferrin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for a variety of research applications.Transferrin is a vital protein involved in iron transport and homeostasis, playing a crucial role in various physiological processes.
Dysregulation of transferrin levels has been linked to conditions such as iron deficiency anemia, hemochromatosis, and inflammatory disorders. Therefore, this kit serves as an essential tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse TRF (Transferrin) CLIA Kit offers researchers a valuable and efficient solution for investigating the role of transferrin in health and disease in mouse models.
Product Name:
Mouse TRF (Transferrin) CLIA Kit
SKU:
MOES00571
Target:
Mouse TRF (Transferrin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TF. You can calculate the concentration of Mouse TF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
87-103
101-115
Average (%)
96
94
109
1:4
Range (%)
87-100
94-107
99-114
Average (%)
93
100
105
1:8
Range (%)
87-101
102-117
99-112
Average (%)
95
109
104
1:16
Range (%)
94-107
87-99
98-110
Average (%)
101
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-116
108
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.33
8.21
1.11
2.49
8.03
Standard deviation
0.12
0.17
0.56
0.1
0.25
0.63
C V (%)
11.21
7.3
6.82
9.01
10.04
7.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TF in samples. No significant cross-reactivity or interference between Mouse TF and analogues was observed.
