The Mouse TRAIL (TNFSF10) ELISA Kit is a powerful tool for the precise measurement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels in mouse serum, plasma, and cell culture supernatants. This kit is designed with optimal sensitivity and specificity for accurate and reproducible results, making it perfect for various research applications.TRAIL is a key protein involved in apoptosis, playing a crucial role in immune response and cell death regulation. Its dysregulation has been linked to cancer, autoimmune diseases, and inflammatory conditions, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions.
Get reliable and insightful data with the Mouse TRAIL (TNFSF10) ELISA Kit to advance your research efforts and uncover new insights into TRAIL biology and its role in disease development. Visit assaygenie.com/mouse-trail-tnfsf10-tumor-necrosis-factor-related-apoptosis-inducing-ligand-elisa-kit-moes01496/ for more information and to order your kit today.
Mouse TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAIL/TNFSF10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRAIL/TNFSF10. You can calculate the concentration of Mouse TRAIL/TNFSF10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
85-99
96-110
Average (%)
92
92
101
1:4
Range (%)
91-101
86-100
87-100
Average (%)
96
92
92
1:8
Range (%)
86-99
82-93
88-101
Average (%)
93
88
93
1:16
Range (%)
86-100
85-97
86-101
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.22
124.92
346.87
49.84
136.7
361.81
Standard deviation
2.69
5.8
11.34
2.82
5.78
13.28
C V (%)
5.36
4.64
3.27
5.66
4.23
3.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TRAIL/TNFSF10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
