The Mouse TPO (Thrombopoietin) CLIA Kit is a powerful tool for the measurement of thrombopoietin levels in mouse samples. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable asset for research studies in a variety of fields.Thrombopoietin is a key regulator of platelet production and plays a critical role in maintaining proper blood clotting function. Dysregulation of thrombopoietin levels has been linked to various hematologic disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
Whether investigating platelet disorders, hematologic diseases, or exploring novel treatment options, the Mouse TPO CLIA Kit offers researchers a reliable and efficient solution for measuring thrombopoietin levels in mouse samples. Trust in the precision and performance of this kit to advance your research efforts and uncover new insights into thrombopoietin biology.
Product Name:
Mouse TPO (Thrombopoietin) CLIA Kit
SKU:
MOES00343
Target:
Mouse TPO (Thrombopoietin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TPO. You can calculate the concentration of Mouse TPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
97-113
85-96
Average (%)
97
103
91
1:4
Range (%)
96-109
100-112
89-102
Average (%)
103
106
97
1:8
Range (%)
91-103
93-109
92-108
Average (%)
98
100
99
1:16
Range (%)
92-108
95-109
86-100
Average (%)
100
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
97-110
105
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.22
24.35
96.16
8.81
23.79
86.65
Standard deviation
0.9
1.73
6.12
0.93
1.68
7.91
C V (%)
9.76
7.1
6.36
10.56
7.06
9.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TPO in samples. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.
