Mouse TPO Recombinant Protein (RPPB1053)
- SKU:
- RPPB1053
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- TPO
- Synonyms:
- Thpo
- C-mpl ligand
- ML
- Megakaryocyte colony-stimulating factor
- Source:
- HEK293 Cells
- Uniprot:
- P40226
Description
| Product Name: | Mouse TPO Recombinant Protein |
| Product Code: | RPPB1053 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | TPO |
| Synonyms: | Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TPO protein (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ?4ng/ml. |
| Amino Acid Sequence: | SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQETHHHHH H |
| Biological Activity: | Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is ? 25ug/ml. |
Thrombopoietin or TPO or MGDF, is a protein (glycoprotein hormone) that can be found in the liver and kidney tissues. TPO regulates the creation of platelets. The protein enhances the production & differentiation of cells such as megakaryocytes that are part of the bone marrow cells that secretes a wide number of platelets. The cellular development process that ends up in the production of platelet calls (Megakaryocytopoiesis). Humoral growth factor is needed for the megakaryocyte proliferation & maturation of megakaryocyte, not apart from thrombopoiesis.
TPO Mouse Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing 341 amino acids ( 22-356 a.a) and having a molecular mass of 36.4kDa. TPO is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation Cellular Component: extracellular space; extracellular region Molecular Function:hormone activity; cytokine activity; receptor binding Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P40226 |
| NCBI GenInfo Identifier: | 730983 |
| NCBI Gene ID: | 21832 |
| NCBI Accession: | P40226.1 |
| UniProt Related Accession: | P40226,JC4227 |
| Molecular Weight: | 37,384 Da |
| NCBI Full Name: | Thrombopoietin |
| NCBI Synonym Full Names: | thrombopoietin |
| NCBI Official Symbol: | Thpo |
| NCBI Official Synonym Symbols: | Ml; Tpo; Mgdf; Mpllg |
| NCBI Protein Information: | thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand |
| UniProt Protein Name: | Thrombopoietin |
| UniProt Synonym Protein Names: | C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand |
| Protein Family: | Thyroid peroxidase |
| UniProt Gene Name: | Thpo |
| UniProt Entry Name: | TPO_MOUSE |
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