The Mouse TNPO1 (Transportin 1) CLIA Kit is specifically designed for the quantitative detection of TNPO1 levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of research applications.TNPO1, also known as Transportin 1, is a critical protein involved in nucleocytoplasmic transport, playing a key role in the transportation of proteins and RNA molecules within the cell.
Dysregulation of TNPO1 has been linked to various diseases, including viral infections and neurological disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Mouse TNPO1 CLIA Kit is an essential tool for researchers looking to explore the function of TNPO1 in cellular transport processes and its implications in health and disease.
Product Name:
Mouse TNPO1 (Transportin 1) CLIA Kit
SKU:
MOES00577
Target:
Mouse TNPO1 (Transportin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNPO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNPO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNPO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNPO1. You can calculate the concentration of Mouse TNPO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
102-117
83-99
Average (%)
95
110
90
1:4
Range (%)
91-107
94-108
96-113
Average (%)
97
101
103
1:8
Range (%)
89-103
102-116
101-116
Average (%)
97
108
109
1:16
Range (%)
97-113
87-99
102-117
Average (%)
104
92
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.4
9.64
0.93
2.33
10.24
Standard deviation
0.1
0.22
0.94
0.12
0.18
0.99
C V (%)
10.1
9.17
9.75
12.9
7.73
9.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNPO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNPO1 in samples. No significant cross-reactivity or interference between Mouse TNPO1 and analogues was observed.
