Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit
The Mouse cTnI (Troponin I, Cardiac) ELISA Kit is specifically designed for the quantitative measurement of cardiac troponin I type 3 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.cTnI is a crucial protein involved in cardiac muscle function, serving as a biomarker for myocardial injury and a key indicator of cardiac health.
Elevated levels of cTnI are associated with conditions such as myocardial infarction, heart failure, and cardiac arrhythmias, making this kit essential for studying these conditions and developing potential therapeutic interventions.With precise and consistent results, the Mouse cTnI ELISA Kit is a valuable tool for researchers studying cardiovascular diseases and seeking to understand the mechanisms underlying cardiac muscle function.
Product Name:
Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit
SKU:
MOES01590
Target:
Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
98-115
92-106
Average (%)
100
105
98
1:4
Range (%)
98-114
82-95
93-106
Average (%)
104
87
99
1:8
Range (%)
98-110
82-94
97-114
Average (%)
104
88
104
1:16
Range (%)
98-111
84-93
97-113
Average (%)
105
88
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.5
62.7
175.1
24.7
60.5
162.0
Standard deviation
1.7
3.0
8.1
1.6
3.1
8.3
C V (%)
6.67
4.78
4.63
6.48
5.12
5.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed.
