The Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily Member 1B) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of TNFRSF1B levels in mouse serum, plasma, and cell culture supernatants. This kit ensures precise and reproducible results, making it an essential tool for a wide range of research applications.TNFRSF1B is a key receptor protein involved in the tumor necrosis factor pathway, playing a crucial role in inflammatory responses and immune regulation.
Dysregulation of TNFRSF1B has been linked to various diseases such as autoimmune disorders, inflammatory conditions, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With its high sensitivity, specificity, and ease of use, the Mouse TNFRSF1B CLIA Kit from Assay Genie provides researchers with a reliable tool for accurate quantification of TNFRSF1B levels, leading to deeper insights into disease mechanisms and novel treatment strategies.
Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNFRSF1B. You can calculate the concentration of Mouse TNFRSF1B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
89-101
95-107
Average (%)
102
94
101
1:4
Range (%)
90-105
91-109
85-99
Average (%)
96
99
91
1:8
Range (%)
97-110
95-110
89-100
Average (%)
103
101
95
1:16
Range (%)
96-109
100-112
94-109
Average (%)
102
105
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
97-112
104
Cell culture media (n=5)
103-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.73
20.0
91.14
9.99
21.21
94.57
Standard deviation
1.34
1.47
7.34
1.22
2.08
8.1
C V (%)
12.49
7.35
8.05
12.21
9.81
8.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNFRSF1B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNFRSF1B in samples. No significant cross-reactivity or interference between Mouse TNFRSF1B and analogues was observed.
