The Mouse TNF-beta (Tumor Necrosis Factor-beta) ELISA Kit is specifically designed for the accurate detection of TNF-beta levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications. TNF-beta is a key cytokine involved in inflammatory responses and immune regulation.
It plays a crucial role in various physiological and pathological processes, including immune response, inflammation, and tissue repair. Detection of TNF-beta levels can provide valuable insights into the pathogenesis of inflammatory diseases, autoimmune disorders, and cancer, making this ELISA kit an essential tool for researchers studying these conditions and developing potential therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-β. You can calculate the concentration of Mouse TNF-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
88-102
93-106
Average (%)
95
94
99
1:4
Range (%)
96-110
87-100
97-110
Average (%)
104
93
104
1:8
Range (%)
95-111
87-102
96-110
Average (%)
102
93
103
1:16
Range (%)
93-110
87-99
90-106
Average (%)
101
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
101
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.1
115.3
372.4
50.6
111.6
385.3
Standard deviation
2.8
6.7
19.0
2.8
6.4
18.9
C V (%)
5.82
5.81
5.1
5.53
5.73
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNF-β in samples. No significant cross-reactivity or interference between Mouse TNF-β and analogues was observed.
