The Mouse TNF-alpha (Tumor Necrosis Factor-alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-alpha levels in mouse samples. This kit is ideal for researchers studying inflammation, immune responses, and various disease models where TNF-alpha plays a critical role.TNF-alpha is a key cytokine involved in inflammatory responses, immune regulation, and cell death pathways. It is implicated in various diseases such as rheumatoid arthritis, inflammatory bowel disease, and sepsis, making it a valuable biomarker for both basic research and clinical studies.
With its high sensitivity and reproducibility, the Mouse TNF-alpha CLIA Kit offers reliable and consistent results, enabling researchers to confidently analyze TNF-alpha expression levels in a variety of sample types including serum, plasma, and tissue culture supernatants. This kit is a powerful tool for advancing our understanding of TNF-alpha biology and developing potential therapeutic strategies targeting this important cytokine.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
98-115
96-114
Average (%)
109
106
104
1:4
Range (%)
88-104
93-105
92-103
Average (%)
95
100
97
1:8
Range (%)
99-113
89-102
86-100
Average (%)
105
95
92
1:16
Range (%)
97-112
90-102
94-112
Average (%)
102
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.22
117.24
278.12
41.21
125.3
276.4
Standard deviation
5.19
11.06
27.45
4.52
11.16
26.7
C V (%)
12.29
9.43
9.87
10.97
8.91
9.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNF-α in samples. No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed.
