The Mouse TN-T (Troponin T) ELISA Kit is specifically designed for the precise and reliable detection of troponin T levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Troponin T is a key protein involved in regulating muscle contraction, particularly in heart muscle cells. Elevated levels of troponin T in the blood are a common marker for heart muscle damage, making this ELISA kit an essential tool for studying cardiovascular diseases and myocardial infarction.
With its advanced technology and superior performance, the Mouse TN-T ELISA Kit from Assay Genie is a valuable asset for researchers looking to investigate troponin T levels in mouse samples and to further understand its role in cardiac health and disease.
Product Name:
Mouse Tn-T (Troponin T) ELISA Kit
SKU:
MOES00688
Target:
Mouse Tn-T (Troponin T)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TnTf/TNNT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TnTf/TNNT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TnTf/TNNT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TnTf/TNNT3. You can calculate the concentration of Mouse TnTf/TNNT3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
100-113
90-104
Average (%)
99
106
95
1:4
Range (%)
101-113
86-98
98-113
Average (%)
107
91
105
1:8
Range (%)
97-113
84-95
94-109
Average (%)
104
89
102
1:16
Range (%)
96-111
82-94
97-109
Average (%)
103
88
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.8
87.0
457.1
53.6
85.3
446.6
Standard deviation
3.6
5.0
23.8
2.7
4.8
21.0
C V (%)
6.82
5.75
5.21
5.04
5.63
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TnTf/TNNT3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TnTf/TNNT3 in samples. No significant cross-reactivity or interference between Mouse TnTf/TNNT3 and analogues was observed.
