The Mouse TN-T (Troponin T) CLIA Kit is specifically designed for the precise measurement of troponin T levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing dependable and consistent results for various research purposes.Troponin T is a critical protein involved in cardiac muscle contraction, serving as a reliable biomarker for myocardial damage and heart-related conditions.
Monitoring troponin T levels can aid in the early diagnosis of heart attacks, myocarditis, and other cardiovascular diseases, making this kit essential for cardiovascular research and clinical applications.
Product Name:
Mouse Tn-T (Troponin T) CLIA Kit
SKU:
MOES00065
Target:
Mouse Tn-T (Troponin T)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TnTf/TNNT3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TnTf/TNNT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TnTf/TNNT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TnTf/TNNT3. You can calculate the concentration of Mouse TnTf/TNNT3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
88-103
86-102
Average (%)
102
96
93
1:4
Range (%)
92-107
95-109
87-102
Average (%)
98
101
94
1:8
Range (%)
94-104
85-99
96-110
Average (%)
99
92
101
1:16
Range (%)
94-108
96-112
86-97
Average (%)
100
104
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
108
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.19
71.94
181.12
24.55
66.01
198.71
Standard deviation
3.12
8.52
18.46
2.6
7.49
12.66
C V (%)
12.39
11.84
10.19
10.59
11.35
6.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TnTf/TNNT3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TnTf/TNNT3 in samples. No significant cross-reactivity or interference between Mouse TnTf/TNNT3 and analogues was observed.
