The Mouse TLN (Talin) CLIA Kit is specifically designed for the precise measurement of talin levels in mouse serum, plasma, and cell culture supernatants. This cutting-edge kit offers unparalleled sensitivity and specificity, guaranteeing consistent and accurate results essential for a variety of research purposes.Talin is a key protein that plays a crucial role in cell adhesion and migration processes, making it a vital component in various physiological and pathological conditions.
By accurately measuring talin levels, researchers can gain valuable insights into cell behavior and potentially identify new therapeutic targets for conditions such as cancer, cardiovascular diseases, and autoimmune disorders.Overall, the Mouse TLN (Talin) CLIA Kit is a powerful tool for elucidating the role of talin in biological processes and advancing research in a wide range of fields.
Product Name:
Mouse TLN (Talin) CLIA Kit
SKU:
MOES00548
Target:
Mouse TLN (Talin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TLN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TLN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TLN. You can calculate the concentration of Mouse TLN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
85-97
89-99
Average (%)
107
90
94
1:4
Range (%)
92-108
100-115
101-115
Average (%)
100
107
108
1:8
Range (%)
97-113
96-112
94-107
Average (%)
103
102
101
1:16
Range (%)
84-95
86-97
90-103
Average (%)
90
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
94-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.08
8.14
1.01
2.12
8.02
Standard deviation
0.09
0.16
0.83
0.12
0.15
0.55
C V (%)
9.47
7.69
10.2
11.88
7.08
6.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TLN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TLN in samples. No significant cross-reactivity or interference between Mouse TLN and analogues was observed.
