The Mouse TJP1 (Tight Junction Protein 1) ELISA Kit is specifically designed for the precise measurement of tight junction protein 1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research applications.Tight junction protein 1 is a key component of tight junctions, which are crucial for maintaining the integrity of epithelial and endothelial cell barriers. Dysregulation of TJP1 has been linked to various diseases, including inflammatory bowel disease, liver diseases, and cancer.
Therefore, this ELISA kit is a valuable tool for studying the role of TJP1 in these conditions and exploring potential therapeutic interventions.Order the Mouse TJP1 ELISA Kit today and unlock the potential of your research in understanding tight junction biology and its implications in health and disease. Visit the website [here](www.assaygenie.com/mouse-tjp1-tight-junction-protein-1-elisa-kit-moes01563/) to learn more and make your purchase.
Product Name:
Mouse TJP1 (Tight Junction Protein 1) ELISA Kit
SKU:
MOES01563
Target:
Mouse TJP1 (Tight Junction Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TJP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TJP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TJP1. You can calculate the concentration of Mouse TJP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
96-114
92-106
Average (%)
102
104
99
1:4
Range (%)
88-103
83-95
87-97
Average (%)
95
88
92
1:8
Range (%)
90-104
83-98
83-92
Average (%)
97
89
87
1:16
Range (%)
86-100
81-91
86-96
Average (%)
93
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
95-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
253.69
766.52
2191.84
238.27
840.02
1992.84
Standard deviation
14.74
37.18
87.89
13.63
35.2
61.78
C V (%)
5.81
4.85
4.01
5.72
4.19
3.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TJP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TJP1 in samples. No significant cross-reactivity or interference between Mouse TJP1 and analogues was observed.
