The Mouse TJP1 (Tight Junction Protein 1) CLIA Kit is specifically designed for the accurate quantification of TJP1 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TJP1 is a key protein involved in the formation and maintenance of tight junctions, which are critical for controlling the passage of molecules across cell layers.
Dysregulation of TJP1 has been implicated in various diseases such as inflammatory bowel disease, cancer, and neurological disorders, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse TJP1 CLIA Kit offers a reliable tool for researchers to accurately measure TJP1 levels in mouse samples, providing valuable insights into the role of tight junctions in health and disease.
Product Name:
Mouse TJP1 (Tight Junction Protein 1) CLIA Kit
SKU:
MOES00564
Target:
Mouse TJP1 (Tight Junction Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TJP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TJP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TJP1. You can calculate the concentration of Mouse TJP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
86-101
84-98
Average (%)
99
93
90
1:4
Range (%)
96-110
88-100
88-100
Average (%)
103
95
93
1:8
Range (%)
100-113
94-108
99-114
Average (%)
106
101
107
1:16
Range (%)
87-98
88-98
101-116
Average (%)
93
93
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.59
85.25
411.05
50.64
92.47
375.7
Standard deviation
5.89
7.77
30.09
6.25
8.83
33.85
C V (%)
12.38
9.11
7.32
12.34
9.55
9.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TJP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TJP1 in samples. No significant cross-reactivity or interference between Mouse TJP1 and analogues was observed.
