Mouse TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
The Mouse TIMP-4 (Tissue Inhibitors of Metalloproteinase-4) CLIA Kit is specifically designed for the detection of TIMP-4 levels in mouse tissue samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for research purposes.TIMP-4 is an important protein that regulates the activity of metalloproteinases, enzymes involved in tissue remodeling and inflammation. Abnormal levels of TIMP-4 have been linked to various diseases, including cancer and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With the Mouse TIMP-4 CLIA Kit, researchers can effectively measure TIMP-4 levels in mouse tissues, advancing our understanding of the role of TIMP-4 in disease progression and potentially identifying new therapeutic targets.
Product Name:
Mouse TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
SKU:
MOES00569
Target:
Mouse TIMP-4 (Tissue Inhibitors of Metalloproteinase 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TIMP-4. You can calculate the concentration of Mouse TIMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
90-105
100-114
Average (%)
96
97
108
1:4
Range (%)
93-107
90-102
96-111
Average (%)
98
97
103
1:8
Range (%)
94-108
87-102
89-103
Average (%)
100
94
95
1:16
Range (%)
91-107
94-106
90-103
Average (%)
99
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-118
107
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.97
152.87
354.65
49.03
158.58
324.25
Standard deviation
5.24
12.43
39.69
5.7
16.35
29.99
C V (%)
10.49
8.13
11.19
11.63
10.31
9.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TIMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TIMP-4 in samples. No significant cross-reactivity or interference between Mouse TIMP-4 and analogues was observed.
