Mouse TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
The Mouse TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TIMP-2 levels in mouse tissue samples. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.TIMP-2 is a key regulator of the extracellular matrix and is involved in processes such as tissue remodeling, cell migration, and angiogenesis.
Dysregulation of TIMP-2 has been implicated in various diseases, including cancer and cardiovascular disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse TIMP-2 CLIA Kit offers researchers a valuable tool for studying the role of TIMP-2 in various biological processes and disease states in mouse models.
Product Name:
Mouse TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit
SKU:
MOES00567
Target:
Mouse TIMP-2 (Tissue Inhibitors of Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TIMP-2. You can calculate the concentration of Mouse TIMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
97-111
89-101
Average (%)
109
103
95
1:4
Range (%)
97-113
93-107
88-102
Average (%)
104
100
94
1:8
Range (%)
95-107
85-98
95-111
Average (%)
100
91
102
1:16
Range (%)
94-110
91-105
96-111
Average (%)
100
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-118
107
EDTA plasma (n=5)
98-113
105
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.91
358.45
1965.64
192.29
372.77
1850.16
Standard deviation
21.12
31.11
185.36
16.34
43.54
147.27
C V (%)
10.41
8.68
9.43
8.5
11.68
7.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TIMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TIMP-2 in samples. No significant cross-reactivity or interference between Mouse TIMP-2 and analogues was observed.
