The Mouse TDGF1 (Teratocarcinoma-Derived Growth Factor 1) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of TDGF1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for a variety of research applications.TDGF1 is an important growth factor involved in embryo development and stem cell maintenance. It plays a crucial role in regulating cell proliferation, differentiation, and survival, making it a key player in various biological processes.
The measurement of TDGF1 levels can provide valuable insight into cellular signaling pathways and potential therapeutic targets for diseases related to stem cell function and development.Overall, the Mouse TDGF1 CLIA Kit offers researchers a powerful tool for studying the role of TDGF1 in various physiological and pathological conditions, allowing for a better understanding of its functions and potential implications for therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TDG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TDG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TDG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TDG1. You can calculate the concentration of Mouse TDG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
97-109
101-117
Average (%)
103
102
108
1:4
Range (%)
86-97
86-98
102-119
Average (%)
91
93
109
1:8
Range (%)
94-108
95-108
84-96
Average (%)
102
102
91
1:16
Range (%)
100-115
93-105
96-111
Average (%)
107
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.99
30.81
76.45
9.49
30.08
77.05
Standard deviation
0.81
3.33
8.65
1.22
3.19
7.68
C V (%)
8.11
10.81
11.31
12.86
10.61
9.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TDG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TDG1 in samples. No significant cross-reactivity or interference between Mouse TDG1 and analogues was observed.
