Mouse TBP2 (Thioredoxin Binding Protein 2) CLIA Kit
The Mouse TBP2 (Thioredoxin Binding Protein 2) CLIA Kit is a reliable and accurate assay designed for the quantitative detection of TBP2 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TBP2 is a key protein that plays a crucial role in redox regulation and has been implicated in various cellular processes, including stress response and cell growth.
Abnormal levels of TBP2 have been associated with multiple diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutics.Overall, the Mouse TBP2 CLIA Kit is a powerful tool for researchers looking to investigate the role of TBP2 in biological processes and disease pathology in mouse models.
Product Name:
Mouse TBP2 (Thioredoxin Binding Protein 2) CLIA Kit
SKU:
MOES00553
Target:
Mouse TBP2 (Thioredoxin Binding Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TBP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TBP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TBP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TBP2. You can calculate the concentration of Mouse TBP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
93-107
91-106
Average (%)
109
101
97
1:4
Range (%)
99-112
88-104
99-112
Average (%)
107
95
104
1:8
Range (%)
86-101
89-101
96-109
Average (%)
93
95
101
1:16
Range (%)
91-103
102-115
97-115
Average (%)
97
107
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.6
604.81
1546.09
177.87
613.69
1643.68
Standard deviation
18.52
59.03
133.74
16.33
60.2
106.84
C V (%)
9.87
9.76
8.65
9.18
9.81
6.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TBP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TBP2 in samples. No significant cross-reactivity or interference between Mouse TBP2 and analogues was observed.
