Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit
The Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit is a specialized assay designed for the precise measurement of thrombin-antithrombin complex levels in mouse serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results, making it an essential tool for researchers studying thrombotic disorders, coagulation processes, and inflammation in mouse models.The thrombin-antithrombin complex is an important marker of coagulation and hemostasis, reflecting the balance between thrombin generation and its inhibition by antithrombin.
Dysregulation of this balance is implicated in various pathological conditions, including thrombosis, disseminated intravascular coagulation, and sepsis. The Mouse TAT ELISA Kit provides researchers with a valuable tool for investigating these conditions and evaluating potential therapeutic interventions.Order the Mouse TAT Thrombin-Antithrombin Complex ELISA Kit today to advance your research in thrombosis, coagulation, and inflammation in mouse models.
Product Name:
Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit
SKU:
MOES01545
Target:
Mouse TAT (Thrombin-Antithrombin Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TAT. You can calculate the concentration of Mouse TAT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
86-102
87-101
Average (%)
98
93
94
1:4
Range (%)
87-99
86-102
83-94
Average (%)
93
93
88
1:8
Range (%)
93-106
81-94
87-101
Average (%)
98
87
93
1:16
Range (%)
92-106
78-92
88-100
Average (%)
98
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.75
156.7
422.93
45.27
169.26
421.6
Standard deviation
2.58
8.52
21.91
2.48
7.41
21.08
C V (%)
5.52
5.44
5.18
5.48
4.38
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TAT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TAT in samples. No significant cross-reactivity or interference between Mouse TAT and analogues was observed.
