Mouse TAT (Thrombin-Antithrombin Complex) CLIA Kit
The Mouse TAT (Thrombin-Antithrombin Complex) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of thrombin-antithrombin complex levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Thrombin-antithrombin complex is an important biomarker of coagulation and inflammation, playing a critical role in the regulation of blood clot formation. Dysregulation of this complex has been implicated in various diseases, including thrombosis, sepsis, and inflammatory disorders.
The Mouse TAT CLIA Kit allows researchers to study the role of thrombin-antithrombin complex in these conditions and potentially develop targeted therapies.Overall, the Mouse TAT CLIA Kit from Assay Genie offers researchers a valuable tool for investigating coagulation pathways and inflammatory processes in mouse models, with the potential to advance our understanding of related diseases and guide the development of novel treatments.
Product Name:
Mouse TAT (Thrombin-Antithrombin Complex) CLIA Kit
SKU:
MOES00558
Target:
Mouse TAT (Thrombin-Antithrombin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TAT. You can calculate the concentration of Mouse TAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
101-114
88-103
Average (%)
92
108
95
1:4
Range (%)
102-113
96-110
95-109
Average (%)
108
101
102
1:8
Range (%)
89-104
99-116
89-101
Average (%)
96
107
94
1:16
Range (%)
86-97
87-97
86-101
Average (%)
92
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.93
67.65
170.05
25.44
64.72
165.83
Standard deviation
2.44
5.66
14.54
2.22
6.76
11.79
C V (%)
10.2
8.37
8.55
8.73
10.44
7.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TAT in samples. No significant cross-reactivity or interference between Mouse TAT and analogues was observed.
