The Mouse SYP (Synaptophysin) ELISA Kit is specifically designed for the precise measurement of synaptophysin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and dependable results, making it perfect for a variety of research purposes.Synaptophysin is a key protein involved in synaptic vesicle trafficking and neurotransmitter release, playing a critical role in neuronal communication and synaptic plasticity. Dysregulation of synaptophysin has been implicated in various neurological disorders such as Alzheimer's disease, Parkinson's disease, and schizophrenia, making it a valuable biomarker for investigating these conditions and developing potential interventions.
Don't miss out on the opportunity to study synaptic transmission and synaptic plasticity with confidence using the Mouse SYP (Synaptophysin) ELISA Kit from Assay Genie. Order yours today at www.assaygenie.com/mouse-syp-synaptophysin-elisa-kit-moes01514/
Product Name:
Mouse SYP (Synaptophysin) ELISA Kit
SKU:
MOES01514
Target:
Mouse SYP (Synaptophysin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SYP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SYP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SYP. You can calculate the concentration of Mouse SYP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
96-110
87-98
Average (%)
101
104
92
1:4
Range (%)
91-104
85-99
81-96
Average (%)
96
92
88
1:8
Range (%)
87-99
87-101
85-98
Average (%)
92
92
90
1:16
Range (%)
93-106
83-95
84-98
Average (%)
99
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.12
4.3
16.61
1.99
4.66
17.34
Standard deviation
0.11
0.18
0.89
0.1
0.21
0.88
C V (%)
5.19
4.19
5.36
5.03
4.51
5.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SYP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SYP in samples. No significant cross-reactivity or interference between Mouse SYP and analogues was observed.
