The Mouse SST (Somatostatin) ELISA Kit is specifically designed for the precise measurement of somatostatin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.Somatostatin is a key hormone that regulates various physiological functions, including inhibiting the release of growth hormone and insulin. It plays a crucial role in the control of cell growth, neurotransmission, and hormone secretion.
Dysregulation of somatostatin is associated with disorders such as acromegaly, diabetes, and certain neurological disorders, highlighting its importance as a biomarker in studying these conditions and potentially developing new therapeutic interventions.Overall, the Mouse SST ELISA Kit provides researchers with a powerful tool to investigate the role of somatostatin in physiological processes and disease pathogenesis, enabling a deeper understanding of its function and potential clinical applications.
Product Name:
Mouse SST (Somatostatin) ELISA Kit
SKU:
MOES01498
Target:
Mouse SST (Somatostatin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SST. During the reaction, SST in the sample or standard competes with a fixed amount of SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SST. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SST in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
99-113
97-112
Average (%)
92
104
105
1:4
Range (%)
85-98
91-104
98-110
Average (%)
91
97
104
1:8
Range (%)
86-101
90-106
101-115
Average (%)
92
98
106
1:16
Range (%)
87-98
95-107
96-112
Average (%)
92
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.5
74.0
196.8
28.7
68.5
190.7
Standard deviation
1.4
4.1
10.2
1.5
2.7
8.4
C V (%)
5.28
5.54
5.18
5.23
3.94
4.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SST concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed.
