Mouse SPA (Pulmonary Surfactant Associated Protein A) CLIA Kit
The Mouse SPA (Pulmonary Surfactant-associated Protein A) CLIA Kit is specifically designed for the accurate measurement of SPA levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SPA is a key protein involved in maintaining proper lung function and surfactant production.
It plays a crucial role in various respiratory conditions, making it a valuable biomarker for studying lung diseases and developing potential therapies.The Mouse SPA CLIA Kit is a valuable tool for researchers studying respiratory health and lung function in mouse models, providing a reliable and efficient way to analyze SPA levels and further understand its role in pulmonary biology.
Product Name:
Mouse SPA (Pulmonary Surfactant Associated Protein A) CLIA Kit
SKU:
MOES00510
Target:
Mouse SPA (Pulmonary Surfactant Associated Protein A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SP-A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SP-A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SP-A1. You can calculate the concentration of Mouse SP-A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-113
90-102
Average (%)
94
103
95
1:4
Range (%)
98-113
95-108
90-105
Average (%)
104
101
96
1:8
Range (%)
100-113
93-105
100-117
Average (%)
106
99
108
1:16
Range (%)
102-119
89-102
93-107
Average (%)
109
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
212.16
605.65
1665.87
224.09
607.18
1592.35
Standard deviation
18.18
43.12
118.94
27.97
44.57
162.1
C V (%)
8.57
7.12
7.14
12.48
7.34
10.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SP-A1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SP-A1 in samples. No significant cross-reactivity or interference between Mouse SP-A1 and analogues was observed.
