Mouse SP-D (Pulmonary Surfactant Associated Protein D) ELISA Kit
The Mouse SP-D (Pulmonary Surfactant-Associated Protein D) ELISA Kit is specifically designed for the accurate measurement of SP-D levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. SP-D is a key protein involved in immune defense in the lungs, playing a critical role in the innate immune response and surfactant function. Dysregulation of SP-D has been associated with various respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and pulmonary fibrosis, making it a valuable biomarker for investigating these conditions and developing potential therapies.
By utilizing the Mouse SP-D ELISA Kit, researchers can gain valuable insights into the role of SP-D in respiratory health and disease, advancing our understanding of lung biology and potential treatment options for respiratory conditions.
Product Name:
Mouse SP-D (Pulmonary Surfactant Associated Protein D) ELISA Kit
SKU:
MOES01432
Target:
Mouse SP-D (Pulmonary Surfactant Associated Protein D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SP-D. You can calculate the concentration of Mouse SP-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
89-105
99-115
Average (%)
99
96
106
1:4
Range (%)
91-104
81-93
84-97
Average (%)
97
87
90
1:8
Range (%)
87-99
84-96
89-102
Average (%)
94
90
94
1:16
Range (%)
92-106
82-94
83-94
Average (%)
98
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.22
3.35
0.5
1.14
3.53
Standard deviation
0.03
0.06
0.11
0.03
0.05
0.17
C V (%)
5.77
4.92
3.28
6.0
4.39
4.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SP-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SP-D in samples. No significant cross-reactivity or interference between Mouse SP-D and analogues was observed.
