The Mouse SOST (Sclerostin) ELISA Kit is designed for the precise measurement of SOST levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing consistent and accurate results for various research applications.Sclerostin, also known as SOST, is a key regulator of bone metabolism, inhibiting bone formation and promoting bone resorption. Dysregulation of SOST levels has been linked to conditions such as osteoporosis, making it a valuable biomarker for studying bone-related disorders and potential therapeutic interventions.
With its reliable performance and ease of use, the Mouse SOST ELISA Kit is an essential tool for researchers investigating the role of SOST in bone health and disease. Get yours today and advance your research with confidence.
Product Name:
Mouse SOST (Sclerostin) ELISA Kit
SKU:
MOES01714
Target:
Mouse SOST (Sclerostin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SOST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SOST. You can calculate the concentration of Mouse SOST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
96-110
96-113
Average (%)
93
101
103
1:4
Range (%)
92-107
82-94
82-96
Average (%)
97
86
88
1:8
Range (%)
88-104
86-97
88-103
Average (%)
95
92
95
1:16
Range (%)
91-104
82-95
84-96
Average (%)
97
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
92-110
100
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.25
122.43
333.91
53.0
125.83
361.81
Standard deviation
3.14
6.78
10.22
3.41
7.13
15.12
C V (%)
6.13
5.54
3.06
6.43
5.67
4.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SOST concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SOST in samples. No significant cross-reactivity or interference between Mouse SOST and analogues was observed.
