The Mouse SNCA (Synuclein Alpha) ELISA Kit is specifically designed for the precise measurement of synuclein alpha levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it a valuable tool for a variety of research applications.Synuclein alpha is a key protein known for its involvement in Parkinson's disease and other neurodegenerative disorders.
It plays a critical role in regulating nerve cell function and is a biomarker of interest for studying these debilitating conditions and advancing potential treatments.With the Mouse SNCA (Synuclein Alpha) ELISA Kit, researchers can confidently analyze and quantify synuclein alpha levels in mouse samples with confidence and precision, ultimately contributing to a deeper understanding of neurodegenerative diseases and the development of therapeutic strategies.
Product Name:
Mouse SNCa (Synuclein, Alpha) ELISA Kit
SKU:
MOES01518
Target:
Mouse SNCa (Synuclein, Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SNCα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SNCα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SNCα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SNCα. You can calculate the concentration of Mouse SNCα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
86-98
88-100
Average (%)
101
91
93
1:4
Range (%)
93-105
84-96
82-95
Average (%)
98
89
88
1:8
Range (%)
86-99
84-94
84-100
Average (%)
93
89
91
1:16
Range (%)
86-99
83-97
85-99
Average (%)
93
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.82
94.36
446.1
46.36
92.27
467.95
Standard deviation
2.65
3.82
16.19
2.86
4.48
23.54
C V (%)
5.32
4.05
3.63
6.17
4.86
5.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SNCα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SNCα in samples. No significant cross-reactivity or interference between Mouse SNCα and analogues was observed.
