The Mouse SNCA (Synuclein Alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of synuclein alpha levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Synuclein alpha is a key protein involved in neurodegenerative disorders such as Parkinson's disease. Its abnormal aggregation is linked to the pathogenesis of these disorders, making it a crucial biomarker for studying disease progression and potential therapeutic interventions.
With its high sensitivity and specificity, the Mouse SNCA (Synuclein Alpha) CLIA Kit is a valuable tool for researchers seeking to better understand the role of synuclein alpha in neurodegenerative diseases and for developing novel treatment strategies.
Product Name:
Mouse SNCa (Synuclein, Alpha) CLIA Kit
SKU:
MOES00545
Target:
Mouse SNCa (Synuclein, Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SNCa. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SNCa and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SNCa, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SNCa. You can calculate the concentration of Mouse SNCa in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
101-115
93-106
Average (%)
109
109
99
1:4
Range (%)
95-109
97-110
85-96
Average (%)
103
102
91
1:8
Range (%)
87-100
102-119
100-113
Average (%)
95
109
106
1:16
Range (%)
88-100
86-96
95-113
Average (%)
95
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.4
56.74
193.51
26.21
55.2
194.53
Standard deviation
2.9
5.51
13.51
2.97
4.33
17.35
C V (%)
10.98
9.71
6.98
11.33
7.84
8.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SNCa concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SNCa in samples. No significant cross-reactivity or interference between Mouse SNCa and analogues was observed.
