The Mouse SLIT2 (Slit Homolog 2) CLIA Kit is a high-quality assay designed for the accurate measurement of SLIT2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SLIT2 is a key protein that plays a critical role in neural development, axon guidance, and cell migration. Dysregulation of SLIT2 has been linked to various neurological disorders and cancers, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and proven performance, the Mouse SLIT2 CLIA Kit is an essential tool for researchers investigating the role of SLIT2 in health and disease. Order yours today and unlock new insights into the complex mechanisms of SLIT2 function.
Product Name:
Mouse Slit2 (Slit Homolog 2) CLIA Kit
SKU:
MOES00448
Target:
Mouse Slit2 (Slit Homolog 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Slit2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Slit2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Slit2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Slit2. You can calculate the concentration of Mouse Slit2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
85-98
94-108
Average (%)
94
91
101
1:4
Range (%)
88-101
84-100
92-105
Average (%)
94
91
99
1:8
Range (%)
100-115
88-102
99-112
Average (%)
107
94
105
1:16
Range (%)
93-111
100-115
97-114
Average (%)
101
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
104
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.81
505.8
1654.53
193.6
479.88
1568.12
Standard deviation
25.42
41.43
132.2
15.64
52.79
126.7
C V (%)
12.85
8.19
7.99
8.08
11.0
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Slit2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Slit2 in samples. No significant cross-reactivity or interference between Mouse Slit2 and analogues was observed.
