The Mouse SLC (Secondary Lymphoid Tissue Chemokine) CLIA Kit is designed for the accurate detection of SLC levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.SLC is a chemokine that plays a crucial role in immune responses by attracting lymphocytes to secondary lymphoid organs. It is involved in various inflammatory and immune-related conditions, making it a valuable biomarker for studying immune system function and responses.
With its high-quality components and user-friendly protocol, the Mouse SLC CLIA Kit provides researchers with a powerful tool for investigating the role of SLC in immune responses, inflammation, and other biological processes. Order now to advance your research and uncover new insights into immune system regulation.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SLC. You can calculate the concentration of Mouse SLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
94-111
88-104
Average (%)
98
102
95
1:4
Range (%)
99-114
99-111
103-116
Average (%)
105
105
110
1:8
Range (%)
99-116
102-116
86-100
Average (%)
107
107
91
1:16
Range (%)
100-113
85-99
100-114
Average (%)
107
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
100-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.18
70.94
239.29
23.32
73.51
244.2
Standard deviation
3.19
6.12
26.97
2.04
7.26
24.13
C V (%)
12.67
8.63
11.27
8.75
9.88
9.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SLC in samples. No significant cross-reactivity or interference between Mouse SLC and analogues was observed.
