The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells. Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8 / BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.
Product Name:
Mouse SLAMF8 Recombinant Protein (RPES0730)
Product Code:
RPES0730
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
SLAM family member 8, B-lymphocyte activator macrophage expressed, CD353, Slamf8, Blame,SBBI42
Accession:
NP_083360.2
Sequence:
Met 1-Asp 231
Fusion tag:
C-His
Activity:
Measured by its ability to bind biotinylated mouse SLAMF8 in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse SLAMF8 (NP_083360.2) extracellular domain (Met 1-Asp 231) was fused with the a polyhistidine tag at the C-terminus.
Purity:
> 80 % as determined by SDS-PAGE
Mol Mass:
24.8 kDa
AP Mol Mass:
33-38 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
